Product category:
Polymerase chain reaction (PCR)
News Release from: Agilent Technologies Europe | Subject: 2100 bioanalyser
Edited by the Laboratorytalk Editorial
Team on 11 April 2002
Semiquantitative detection of gene
expression
New method allows semi-quantitative gene expression to be achieved, substituting or even replacing real-time PCR for certain applications
Agilent Technologies Europe has announced a new application for the Agilent 2100 bioanalyser in conjunction with the DNA 1000 LabChip kit for the semiquantitation of mRNA expression The research on the quantitation of mRNA expression was conducted at the Forschungszentrum in Karlsruhe, Germany and took advantage of comparative multiplex reverse transcription PCR
This article was originally published on Laboratorytalk on 21 Feb 2008 at 8.00am (UK)
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"Generating data with the Agilent 2100 bioanalyser is one of the fastest and most robust methods for the analysis of quantitative or semiquantitative PCR applications I have experienced to date," said Eric Gottwald of the Forschungszentrum in Karlsruhe.
For this application, the primer dropping method, first described in 1994 by Wong et al, was employed.
Until recently comparative PCR experiments depended on gel analysis, a slow, manual technique that does not provide accurate quantitation for multiple PCR.
The DNA 1000 assay used with the Agilent 2100 bioanalyser delivers accurate quantitation of multiple products over a wide range of concentration.
Using this method semi-quantitative gene expression can be achieved, substituting or even replacing real-time PCR for certain applications.
In contrast to real-time PCR, a multitude of genes can be analysed in one single reaction.
For further information, request application note 'Semiquantitative reverse transcription-polymerase chain reaction with the Agilent 2100 Bioanalyser', publication number 5988-4556EN.
This application note is available without charge from any Agilent sales office or from its website (details above).
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