Product category:
Genomics
News Release from: Agilent Technologies Europe | Subject: Oligonucleotide libraries
Edited by the Laboratorytalk Editorial
Team on 21 February 2008
A new generation of gene-silencing
libraries
These libraries contain constructs against all identified genes in the human and mouse genomes with the unique ability to silence targeted genes specifically and effectively over long time periods
Agilent Technologies announces an agreement to provide the Hannon laboratory at Cold Spring Harbor Laboratory (CSHL) with oligonucleotide libraries to further develop the widely used small hairpin RNA interference (shRNA) libraries Howard Hughes Medical Institute investigators Gregory Hannon, at CSHL, and Stephen Elledge, the Gregor Mendel professor of Genetics at Harvard Medical School, pioneered the generation of plasmid-based shRNA libraries
This article was originally published on Laboratorytalk on 15 Aug 2001 at 8.00am (UK)
Related stories
Discount on GC and GC/MS systems
Agilent Technologies Europe has announced a 15% discount on new gas chromatograph (GC) and gas chromatograph/mass selective (GC/MS) detection systems purchased before 16 December 2001
'Separation Times' lives on
Agilent Technologies has published the latest edition of the magazine Separation Times, following its acquisition of the previous publisher JandW Scientific
The earlier collaborative work was published in Nature Methods 1, 241-248 (2004) and Nature Genetics 37, 1281-1288 (2005).
"Agilent has significantly improved its programmable in-situ oligonucleotide synthesis technology since the original publication, and we've developed the capability to synthesize as many as 55,000 oligonucleotides per library, and also make them as long as 200 bases," said Emily LeProust, R+D chemistry manager, genomics for Agilent.
"This means that we can provide Dr Hannon with high quality starting material in a massively parallel fashion and at a cost significantly lower than current oligonucleotide synthesis technology.
"The 100 base-pair barrier has been broken, and researchers no longer need to combine individually synthesised oligos from microplates into a single tube".
Agilent says that it is producing at a very low error rate (one out of 250 bases or better), and is delivering quantities from 0.2fmol to tens of fmols per oligo.
The flexible synthesis platform permits rapid prototyping of an oligo mixture prior to scale-up for larger studies.
The ability to synthesise long oligos of high quality enables novel approaches to the study of biological problems and systems.
Agilent oligo libraries are available to selected users under an early access programme.
• Agilent Technologies Europe: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
