Product category:
Nucleic acid sequencing and synthesis
News Release from: Ambion (Europe) | Subject: EndoFree RT
Edited by the Laboratorytalk Editorial
Team on 14 September 2001
Eliminate nonspecific priming in cDNA
synthesis
Ambion's new EndoFree RT kit is the first reverse transcription kit engineered to eliminate unwanted endogenous random priming that occurs in standard cDNA synthesis reactions
hen using gene-specific or oligo(dT) primers, the EndoFree RT kit will increase the specificity of cDNA labelling for array analysis, improve template preparation for aRNA amplification, and enhance any application that requires specific priming during first-strand synthesis A significant amount of non-specific priming occurs in reverse transcription reactions performed under standard conditions, regardless of which primers are used to prime the RT reaction
This article was originally published on Laboratorytalk on 10 Jul 2001 at 8.00am (UK)
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Nonspecific priming will lead to lowered signal in downstream assays that require a specific sequence be added to a synthesised cDNA, or when priming needs to be initiated at a sequence-specific site.
For applications that depend on synthesis of specific products eliminating endogenous priming can greatly increase experimental sensitivity and specificity.
The EndoFree RT kit comes complete with everything needed for 25 cDNA synthesis reactions, including enzymes, reagents, oligo(dT) first-strand primer and a detailed instruction manual.
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