Product category:
Proteomics
News Release from: AnaSpec | Subject: Phosphopeptide Antibody Package
Edited by the Laboratorytalk Editorial
Team on 21 June 2006
Custom phosphospecific antibody
production service
The production of antibodies against phosphopeptides has often presented a challenge for researchers in search of high-quality results
Much of the challenge has been attributed to the finicky stability of the phosphorylation on the peptide AnaSpec, a provider of integrated proteomics solutions, believes that its expertise in synthesizing phosphorylated peptides will be crucial in answering the need for high-quality phosphospecific antibodies
This article was originally published on Laboratorytalk on 7 Jun 2006 at 8.00am (UK)
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Cellular phosphorylation is a reversible covalent modification of a protein or lipid that modifies the activity of the phosphorylated molecule by inducing conformational changes within the molecule
As a result, AnaSpec now offers custom phosphospecific antibody production as one of its major antibody services.
AnaSpec offers a complete Phosphopeptide Antibody Package that includes: 15mg (90% purity, up to 15 amino acids) phosphospecific peptide - 5 mg delivered to researcher, 10 mg for antibody production.
11mg (80% purity, up to 15 amino acids) non-phosphospecific peptide - used for affinity depletion (in-house only).
Immunisation of two rabbits.
Phosphospecific and non-phosphospecific peptide Elisa titration of antibody for all bleeds.
Pre-immune sample of serum (5ml/each rabbit) and anti-serum (100ml total).
Affinity purification of selected batch of serum.
Shipment of purified phosphospecific antibody which does not cross-react with non-phosphospecific peptides (as confirmed by Elisa).
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