Product category:
Proteomics
News Release from: AnaSpec | Subject: HCV NS3/4A protease Fret substrate
Edited by the Laboratorytalk Editorial
Team on 13 July 2006
Ultra-sensitive HCV NS3/4A protease Fret
substrate
Applying both peptide and fluorescent dye technologies, AnaSpec has developed an ultra-sensitive HCV NS3/4A protease Fret substrate that can detect presence of HCV NS3/4A protease at <0.1pmol
NS3/4A protease is the protease responsible for the cleavage of HCV nonstructural polyprotein at the NS3-NS4A, NS4A-NS4B, NS4B-NS5A, and NS5A-NS5B sites Since these cleavages are essential for the maturation of the viral proteins, this protease has become one of the key targets for developing anti-HCV drugs
This article was originally published on Laboratorytalk on 7 Jun 2006 at 8.00am (UK)
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Reagents kits for study of protein phosphorylation
Cellular phosphorylation is a reversible covalent modification of a protein or lipid that modifies the activity of the phosphorylated molecule by inducing conformational changes within the molecule
Focusing on this particular protease, AnaSpec has developed an innovative new tool for optimised detection performance.
Applying both peptide and fluorescent dye technologies, AnaSpec has developed an ultra-sensitive HCV NS3/4A protease Fret substrate that can detect the presence of HCV NS3/4A protease at levels of <0.1pmol.
Based on the peptide sequence, Ac-DED(EDANS)EE-Abu-[y,COO]ASK(DABCYL)-NH2, developed by Taliani et al, the donor and the quencher have been substituted with 5-Fam and the company's trademark quencher QXL 520, respectively.
This substrate, Ac-DE-DAP(QXL520)-EE-Abu-[y,COO]AS-C(5-FAMsp)-NH2, shows a 22-fold lower Km than the EDANS/DABCYL peptide.
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