Product category:
Proteomics
News Release from: AnaSpec | Subject: Haba Biotin quantitation kits
Edited by the Laboratorytalk Editorial
Team on 24 January 2008
AnaSpec introduces Haba-biotin
quantitation kits
Haba Biotin quantitation kits provide a convenient method for estimating the molar ratio of biotin to protein on biotinylated conjugates
AnaSpec's new line of Haba Biotin quantitation kits can also be used to quantitate the biotin concentration in a solution Available in two sizes:
This article was originally published on Laboratorytalk on 7 Jun 2006 at 8.00am (UK)
Related stories
Reagents kits for study of protein phosphorylation
Cellular phosphorylation is a reversible covalent modification of a protein or lipid that modifies the activity of the phosphorylated molecule by inducing conformational changes within the molecule
120 Assays 72096-120 (120 assays in a 96-well format or 25 assays in a cuvette format).
500 Assays 72096-500 (500 assays in a 96-well format or 100 assays in a cuvette format).
Biotin and avidin (or streptavidin) bind non-covalently with a higher binding affinity than most antigen-antibody interactions.
This very tight binding makes labelling proteins with biotin a useful tool for applications such as affinity chromatography and immunoanalytical assays.
The assay utilises the observation that Haba (4'-hydroxyazobenzene-2-carboxylic acid) shows dramatic spectral changes when it binds to avidin.
Free Haba has an absorption peak at 348nm, while the Haba/avidin complex has strong absorption at 500nm.
Since the affinity between Haba and avidin is relatively weak (Kd=5.8 x10-6 M) compared to the affinity between biotin and avidin (Kd=1 x10-15 M), biotin can easily replace Haba from the Haba/avidin complex, resulting in a decrease of absorption at 500 nm.
Avidin and Haba at an optimal ratio are included in the assay kit.
The kit has a linear range of 2-16mM.
• AnaSpec: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
