Anaspec enhances alkaline phosphatase assays

Edited by the Laboratorytalk editorial team Nov 11, 2009

Anaspec has announced that the Sensolyte PNPP alkaline phosphatase assay kits now contain a specially formulated PNPP substrate.

This substrate is ready to use as there is no need to reconstitute PNPP powder.

It offers improved sensitivity - detecting as low as 0.02ng alkaline phosphatase.

It also offers improved stability - storing at 4C for one year.

Changes in alkaline phosphatase level and activity are involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation-related diseases, inflammatory bowel disease, post-parathyroidectomy stage and drug toxicity.

Alkaline phosphatase is widely used in Elisa for conjugation with secondary antibody and as a reporter for gene expression studies.

These Sensolyte PNPP alkaline phosphatase assay kits provide a colorimetric assay for detecting alkaline phosphatase in biological samples and in Elisa with alkaline phosphatase conjugated secondary antibody or streptavidin by using colorimetric PNPP (p-Nitrophenyl phosphate) phosphatase substrate.

Upon dephosphorylation, PNPP turns yellow and can be detected at an absorbance of 405nm.

The Sensolyte PNPP alkaline phosphatase Elisa assay kits provide an AP-conjugated secondary antibody - goat anti-mouse (cat: 72147-M) or goat anti-rabbit (cat: 72147-R).

Anaspec also offers a PNPP assay kit for secreted alkaline phosphatase reporter assay, the Sensolyte PNPP secreted alkaline phosphatase (SEAP) reporter gene assay kit.

A luminometric SEAP kit is also available.