Isolation and analysis of protein-based biomarkers

Liquid chromatography and spin columns selectively partition the 12 most abundant proteins from human serum or plasma, ennriching the proteome to provide primary targets for biomarker discovery

Beckman Coulter introduces a new family of ProteomeLab partitioning systems and chemistries, providing advanced sample preparation for the isolation and analysis of protein-based biomarkers.

The first product in this new line is the ProteomeLab IgY-12 proteome partitioning chemistry.

Based on avian-generated antibodies bound to inert beads, ProteomeLab IgY-12 liquid chromatography and spin columns selectively partition the 12 most abundant proteins - up to 96% of the protein mass - from human serum or plasma.

The enriched proteome, which includes medium- and low- abundant proteins, is the primary target for biomarker discovery.

This material can then be collected and further fractionated with Beckman Coulter's ProteomeLab PF 2D system.

IgY chemistries offer cleaner capture and broader antigen-binding host range than other capture methods because of the evolutionary distance between chickens and mammals.

Whereas alternative plasma depletion methods only remove between one and six abundant proteins, the new ProteomeLab IgY-12 chemistry specifically partitions 12 highly abundant proteins that account for up to 96% of the protein mass.

"Highly abundant proteins significantly mask potential biomarkers," explained Jeff Chapman, director of Beckman Coulter's proteomics business centre.

"Digging deeper into the proteome by removing 12 highly abundant proteins vastly enhances discovery and validation of biomarkers, drug targets and animal models".

The IgY proteome partitioning chemistry is packaged in three formats: a high-capacity LC column that can yield 3-5mg of enriched proteome per day, a medium-capacity LC column that can yield 0.6-1mg per day, and spin columns that can yield 0.3-0.5mg per day.

These products are available as complete solutions including validated methodology, the necessary reagents and instrumentation.

Back to top