Product category:
Nucleic acid sequencing and synthesis
News Release from: Bio-Rad Laboratories | Subject: Immun-Star HRP kit
Edited by the Laboratorytalk Editorial
Team on 20 December 2002
HRP chemiluminescent kit is highly
sensitive
Bio-Rad has expanded its Immun-Star line of chemiluminescent kits for western blot detection to include the Immun-Star HRP kit for horseradish peroxidase (HRP) conjugated secondary antibodies.
Bio-Rad has expanded its Immun-Star line of chemiluminescent kits for western blot detection to include the Immun-Star HRP kit for horseradish peroxidase (HRP) conjugated secondary antibodies This highly-sensitive, fast-acting alternative to isotopic detection is able to detect less than one picogram of sample, and the light signal lasts 6-8 hours after activation
This article was originally published on Laboratorytalk on 19 Feb 2002 at 8.00am (UK)
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Successful experiments can be run with small amounts of sample, and there's no need to rush into the darkroom after activation.
Working solutions are stable at room temperature for 24 hours, and kitsū shelf life is one year at 4deg C.
Film exposures are as low as 30 seconds to 2 minutes on commonly used blotting supports.
These superior light signals also produce excellent, quantifiable results on imaging systems such as Bio-Rad's ChemiDoc and VersaDoc systems.
The chemical reaction of Immun-Star HRP involves a peroxidase-catalyzed oxidation of luminol which produces light.
The addition of an enhancer, which is included in the kit, intensifies the light signal and increases its duration.
If desired, most blots can be reused by stripping and reprobing.
Immun-Star HRP kits are available in configurations to suit customer's precise needs, including: substrate only, substrate with conjugate and substrate with conjugate and blotting reagents. Request a free brochure from Bio-Rad Laboratories ...
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