Product category:
Nucleic acid sequencing and synthesis
News Release from: Bio-Rad Laboratories | Subject: iTaq Supermix with Rox
Edited by the Laboratorytalk Editorial
Team on 24 November 2004
PCR supermix for probe-based detection
Formulation gives high performance with cDNA as well as genomic and plasmid DNA templates over a broad dynamic range, achieving sensitive and specific amplification
The iTaq Supermix with Rox is a new real-time PCR supermix designed for probe-based detection on all Rox-dependent optical thermal cyclers The formulation gives high performance with cDNA as well as genomic and plasmid DNA templates over a broad dynamic range, achieving sensitive and specific amplification over at least seven orders of magnitude, says Bio-Rad The supermix is pre-blended with hot-start iTaq DNA polymerase, optimised buffer, nucleotides including dUTP, and the passive reference dye Rox
This article was originally published on Laboratorytalk on 19 Feb 2002 at 8.00am (UK)
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Its convenient one-tube formulation enables users to obtain sensitive and specific amplification every time.
Features of this novel formulation of real-time PCR include the following:.
Contains the passive reference dye Rox for normalisation of fluorescent signals and well-to-well optical variations.
All reaction components are pre-blended for ease of use on all Rox-dependent platforms and easy set-up and storage.
Unique low foam formulation minimises bubble formation during pipetting, thereby improving accuracy.
Contains hot-start iTaq DNA polymerase for stringent PCR amplification.
Supermix incorporates dUTP for optional UNG pre-treatment of possible contaminants. Request a free brochure from Bio-Rad Laboratories ...
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