Product category:
Nucleic acid sequencing and synthesis
News Release from: Bio-Rad Laboratories | Subject: Technical bulletin 5362
Edited by the Laboratorytalk Editorial
Team on 30 October 2007
Minimise PCR times using conventional
instruments
Bio-Rad Laboratories latest technical bulletin (bulletin 5362) describes how to shorten PCR run times by up to 50% using conventional PCR instruments, reagents, and reaction vessels
Although many researchers in the life, pharmaceutical, and forensic sciences may believe that fast PCR is only obtainable through the purchase of specialised and faster-ramping thermal cyclers, the new tech bulletin explains how most of the time savings can be achieved by simply modifying thermal cycling conditions By following the guidance detailed in the tech bulletin, it is possible to:
This article was originally published on Laboratorytalk on 19 Feb 2002 at 8.00am (UK)
Related stories
Fully automated BSE testing system
New tests and automated platform promise to increase efficiency, produce faster results, expand mass screening capabilities, and reduce costs
* Shorten run times for standard PCR from approximately 90 to 35 minutes.
* Reliably amplify long targets (1-20kb) three to four times faster than standard protocols.
* Obtain real-time quantitative PCR (qPCR) data with Sybr Green or TaqMan chemistries in less than an hour.
The six-page tech bulletin includes diagrams, tables, and graphics that show how one can improve run-time for each of the steps involved a conventional PCR run process.
The bulletin also shows how one can shorten the run-time of real-time qPCR and save time in amplifying long targets.
The guide concludes with an addendum that provides general considerations for fast-PCR, the rapid optimisation strategy for fast-PCR, and troubleshooting tips.
Bulletin 5362 is available either from a local Bio-Rad sales office or it may be downloaded. Request a free brochure from Bio-Rad Laboratories ...
• Bio-Rad Laboratories: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
