Product category:
Nucleic acid sequencing and synthesis
News Release from: Bio-Rad Laboratories | Subject: ProteoMiner
Edited by the Laboratorytalk Editorial
Team on 22 November 2007
Uncover low-abundance proteins in
complex samples
ProteoMiner protein enrichment kits employ a novel bead-based technology, using combinatorial peptide ligands to simultaneously dilute high-abundance proteins and concentrate low-abundance proteins
Bio-Rad Laboratories has introduced its new ProteoMiner protein enrichments kits, which enable researchers to concentrate and enrich low- and medium-abundance proteins that cannot be detected through traditional methods ProteoMiner protein enrichment kits are compatible with a variety of sample types and can be used with most downstream protein analysis techniques including one- or two-dimensional gel electrophoresis, chromatography, and surface-enhanced laser desorption/ionisation
This article was originally published on Laboratorytalk on 10 Dec 2007 at 8.00am (UK)
Related stories
Notes on ProteoMiner protein enrichment kit
Bio-Rad application note, bulletin 5632, provides information on the ability of ProteoMiner protein enrichment kits to concentrate and enrich low- and medium-abundance proteins for biomarker discovery
Concentrate low-and medium-abundance proteins
ProteoMiner kits are compatible with a variety of sample types and can be used with all major downstream protein analysis techniques including 1D or 2D gel electrophoresis and mass spectrometry
"ProteoMiner kits enable life and pharmaceutical researchers to reduce the complexity of biological samples and detect low-abundance proteins," said Kate Smith, Bio-Rad's expression proteomics product manager.
When a complex biological sample is applied to the beads, high-abundance proteins saturate their high affinity ligand(s), and excess protein is washed away.
In contrast, low-abundance proteins are concentrated on their specific affinity ligand(s).
To provide flexibility for use with multiple downstream applications, two ProteoMiner protein enrichment kit formats are available.
Both are optimised for use with serum and plasma samples, but they may also be used with other sample types with at least 50mg total protein available.
The ProteoMiner protein enrichment introductory kit, provides columns and all necessary reagents for accessing low-abundance proteins in a variety of biological samples and is compatible with the majority of downstream proteomics applications.
This kit can be used to process two samples.
The full-size kit can be used to process up to ten samples.
The ProteoMiner sequential elution kit is available for researchers who wish to elute their proteins into multiple fractions to detect additional proteins.
This kit has been optimised for Seldi experiments and provides columns and reagents for processing up to ten samples. Request a free brochure from Bio-Rad Laboratories ...
• Bio-Rad Laboratories: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
