Product category:
Proteomics
News Release from: Bio-Rad Laboratories | Subject: Profinity eXact
Edited by the Laboratorytalk Editorial
Team on 27 February 2008
Fast forward to tag-free protein
Bio-Rad's new Profinity eXact fusion-tag system is an integrated set of products that address bottlenecks in the tag-removal process involved with the purification of affinity-tagged proteins
The Profinity eXact fusion-tag system utilises an immobilised, engineered Subtilisin protease to carry out affinity binding and tag cleavage, which significantly shortens the purification process As the ligand, the protease is not only involved with the binding and recognition of the tag, but upon application of the elution buffer, it also serves to quickly and precisely cleave the tag from the fusion protein directly after the cleavage recognition sequence
This article was originally published on Laboratorytalk on 19 Feb 2002 at 8.00am (UK)
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This delivers a native, tag-free protein in a single step.
The result of the entire process is true, single-step purification without the difficulty and expense of cleavage enzymes, incubation times, or removal of reagents.
"Profinity eXact enables researchers to purify tag-free proteins in one hour," said Tanis Correa, senior product manager of Bio-Rad lab chromatography products.
"It's an excellent option for scientists utilising the simplicity of affinity purification, but whose applications demand unadulterated protein for functional and structural analyses".
The key benefits of the Profinity eXact system include purification and processing of fusion-tagged proteins in a single step, on-column cleavage in as little as 30 minutes, elimination of a protease addition step, and precise cleavage at the N-terminus to generate a native protein sequence.
Bio-Rad's Profinity eXact Fusion-tag System is available immediately. Request a free brochure from Bio-Rad Laboratories ...
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