Product category:
Proteomics
News Release from: Bio-Rad Laboratories | Subject: Technical note 5513A
Edited by the Laboratorytalk Editorial
Team on 31 March 2008
Protein purification: automatic vs
manual
Bio-Rad Laboratories's technical note 5513A compares the performance and reproducibility of the automated Profinia protein purification system to traditional manual gravity-flow column purification
To enable a valid comparison, the B-PER GST fusion protein purification kit from Pierce Biotechnology was chosen, and identical application and elution volumes were used for the columns tested between the two systems During the test, unlike the manual gravity-flow purification kit, the Profinia system did not require user handling between the application of the sample and the conclusion of the affinity purification and desalting steps
This article was originally published on Laboratorytalk on 19 Feb 2002 at 8.00am (UK)
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Those who conducted the test concluded that reduced sample handling and hands-on time were the principal benefits over manual purification.
The Profinia system was superior in protein yield and reproducibility and was equivalent to the manual system in terms of protein purity.
One key feature of the Profinia system is the automated collection of purification data, an option that is not available with the manual system.
The automated collection of purification data simplifies the evaluation of the effectiveness of purification and diagnostic purpose for trouble shooting.
These data are captured in a report that contains chromatograms, continuous records of various method parameters, yield, volumes, and purification conditions.
Another key feature discussed in the bulletin is the integration of the desalting step into the purification step.
The immediate automated desalting after affinity purification not only saves time but also provides ready-to-use protein for downstream assays. Request a free brochure from Bio-Rad Laboratories ...
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