Paper describes how RNA degradation affects data
A
Bio-Rad Laboratories product story
Edited by the Laboratorytalk editorial team
Nov 27, 2008
Bio-Rad Laboratories has updated bulletin 5452, which describes the effect of RNA degradation on data quality in quantitative PCR and microarray experiments.
The paper discusses the use of automated electrophoresis in determining RNA quality prior to gene expression analysis.
Quantitative expression levels, when determined by qPCR, decrease as RNA degrades.
This can lead to erroneous conclusions regarding the levels of gene expression when comparing samples degraded to different extents.
Genes with high levels of expression can still be characterised but degradation compromises the ability to detect differences in genes expressed at low levels.
Bio-Rad's Experion automated electrophoresis system determines the quality and quantity of RNA in gene expression analysis experiments.
It accurately quantitates and evaluates the integrity of samples using as little as 200pg total RNA, which is several hundred times less material than required for gel electrophoresis.
Once RNA quantity and quality have been established, a researcher can decide whether to move forward with additional experiments, knowing to what extent the results may be compromised.
The six-page paper provides a detailed introduction, methods description, results and a discussion, supported by numerous graphs, tables, figures, and electropherograms.
Bulletin 5452 is available from any Bio-Rad sales office or from the Bio-Rad website.
Type 5452 into the search box in the top-right corner of the home page to obtain a copy.
Not what you're looking for? Search the site.
Contact Bio-Rad Laboratories
Tel +44 20 8328 2000
Other Bio-Rad Laboratories stories
-
Note describes new RQI for Experion
Bio-Rad Laboratories has announced the availability of a technical note that describes a new RNA quality indicator (RQI) available with the company's Experion automated electrophoresis system. -
Bio-rad releases Experion software
Bio-rad Laboratories has announced the release of software for its automated Experion electrophoresis system, which enables high resolution results for protein, RNA, and DNA separations. -
Better sensitivity in SDS-protein electrophoresis
Bio-Rad Laboratories has released a technical note (tech note 5719A) entitled: Experion automated electrophoresis system: enhancing sensitivity in SDS-protein electrophoresis -
Chromatographic method monitoring technical note
Technical note demonstrates how the Experion and Pro260 kit can be used to accelerate the process of developing a purification scheme for an antibody or other protein -
RNA quantitation performance: methods compared
Bio-Rad Laboratories reports the availability of a technical paper, bulletin 5420A, that compares the performance of Bio-Rad's Experion system to other methods of RNA quantitation
Articles by product category
-
Analytical instruments (8,805)
- Analytical instruments (13)
- Chemical analysis equipment (631)
- Chromatographs: gas (241)
- Chromatographs: liquid, ion, gel, HPLC (427)
- Chromatography accessories (503)
- Gas analysis equipment (219)
- pH and conductivity meters (114)
- Cameras and imaging systems (820)
- Electron microscopy systems (209)
- Optical microscopes (448)
- Microscope supplies and accessories (558)
- Mass spectrometers (458)
- Spectroscopy (535)
- Other analytical instruments (592)
- Particle size analysis equipment (555)
- Microplate readers (39)
- Temperature, pressure and flow measurement (262)
- Spectrophotometers (273)
- Titration equipment (655)
- Ultrasound equipment and ultrasonic spectroscopy (118)
- Viscometers and rheometers (346)
- Oscilloscopes (83)
- Mechanical testing equipment (492)
- Test and calibration instrumentation (214)
-
Life sciences and clinical laboratory equipment (3,579)
- Life sciences and clinical laboratory equipment (3)
- Clinical chemistry analysis (898)
- Nucleic acid sequencing and synthesis (637)
- Genomics (459)
- Proteomics (450)
- Assay kits (185)
- Microbiology (383)
- Polymerase chain reaction (PCR) (240)
- Cell/tissue handling and counting (324)
-
Computer hardware (826)
- Computer hardware (7)
- Dataloggers (302)
- I/O devices (362)
- Monitors and peripherals (50)
- Process control and networking (79)
- PLCs (26)
-
Industry news (19)
- Industry news (19)
-
Laboratory equipment and wares (5,937)
- Laboratory equipment and wares (5)
- Safety and ventilation equipment (322)
- General lab equipment (831)
- Workstations (61)
- Detectors, sensors and probes (375)
- Lab automation equipment and robotics (651)
- Laboratory furniture and storage (151)
- Gas handling (125)
- Heating: ovens, furnaces, autoclaves (331)
- Fermentation and incubation equipment (73)
- Microplates/microarrays and readers (397)
- Microfluidics/Lab-on-a-chip systems (142)
- Distillation equipment (16)
- Sampling equipment (156)
- Environmental chambers (88)
- Fume cupboards ductless (79)
- Laboratory glassware (400)
- Liquid handling (415)
- Cooling: refrigeration (198)
- Washing and sterilisation equipment (207)
- Fume cupboards ducted (31)
- HTS and combinatorial chemistry equipment (57)
- Evaporation equipment (166)
- Weighing and balancing (240)
- Centrifuges (47)
- Water purification (213)
- Laboratory plasticware (160)
-
Laboratory supplies and consumables (1,271)
- Laboratory supplies and consumables (2)
- Culture media (119)
- Reagents and catalysts (169)
- Antibodies (203)
- Cleaning products (65)
- Reference standards (178)
- Chemicals and biochemicals (534)
-
Pumps, Valves, Filters (708)
- Automated Valves (66)
- Filtration equipment and supplies (176)
- Metering pumps (109)
- Other pumps (63)
- Peristaltic pumps (65)
- Vacuum pumps and compressors (144)
- Manual Valves (85)
-
Services (2,989)
- Contract research (889)
- Laboratory and scientific exhibitions and conferences (230)
- Laboratory and scientific recruitment (26)
- Laboratory and scientific consultancy services (288)
- Laboratory and scientific training and education (413)
- Laboratory building and design (50)
- Laboratory and scientific manufacturing services (136)
- Laboratory and scientific professional organisations (482)
- Laboratory and scientific books and publishing (475)
-
Computer software (2,882)
- Computer software (4)
- Chemical modelling software (181)
- Laboratory and scientific databases (327)
- Instrument control software (320)
- Laboratory information management system (LIMS) (721)
- Mathematical software (163)
- Process control and networking software (52)
- Data acquisition software (171)
- Chromatography software (284)
- Spectroscopy software (92)
- Analytical, statistical and report generating software (284)
- Data management software (283)
