Product category:
Chromatographs: liquid, ion, gel, HPLC
News Release from: Dionex (UK) | Subject: Dual Gradient UltiMate
Edited by the Laboratorytalk Editorial
Team on 01 April 2003
Parallel capillary/nano LC separations
Dual gradient system provides powerful identification of peptides for pharmaceutical, research and other applications and can increse throughput by 100%
Dionex has introduced a new dual gradient capillary/nano LC system that allows for parallel capillary/nano LC separations The dual gradient system provides powerful identification of peptides for pharmaceutical, research and other applications
This article was originally published on Laboratorytalk on 28 May 2008 at 8.00am (UK)
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By coupling this system to NanoSpray/MS, the throughput of the MS can be increased by about 100%.
The system consists of two micropumps, both capable of gradient flow rates as low as 50nL/min.
A typical nano LC separations consists of a 30min gradient, with 10 to 15 minutes for equilibration and 0 to 5 minutes for sample loading.
Analytes such as peptides are eluted only during the course of the gradient, usually 10-50% of the organic modifier (eg, acetonitrile).
This reduces the total MS analysis time by about 50% for the identification of peptides.
Using the dual gradient UltiMate System, the time traditionally required for loading and equilibration can be used for running a second sample, and the time traditionally required for MS acquisition is no longer a consideration.
Consequently, MS throughput can increase up to 100%. Request a free brochure from Dionex (UK) ...
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