Product category:
Clinical chemistry analysis
News Release from: ESA Biosciences | Subject: Choline HPLC Test
Edited by the Laboratorytalk Editorial
Team on 13 June 2007
Validated choline measurement in blood
or plasma
The test is the first validated choline assay on the market suitable for use as a reference test and for large-scale clinical- and epidemiological-research studies
ESA Biosciences has developed an easy, fast, and specific choline test, for research use only The assay, which can be run on either the ESA Coulochem or CoulArray HPLC system, accurately quantitates choline in whole blood or plasma in just 4.5minutes
This article was originally published on Laboratorytalk on 24 Jul 2008 at 8.00am (UK)
Related stories
Determining low level impurities in APIs
ESA Biosciences has a new technical poster describing how the Corona Cad enables routine measurement of low-nanogram levels of organic impurities in active pharmaceutical ingredients
Free guide to optimised electrochemical detection
ESA Biosciences has published an informative 14-page guide for laboratories interested in gaining the full sensitivity and selectivity benefits from a HPLC electrochemical detection (ECD) system
An essential nutrient that plays many key roles in the body, choline is gaining increased attention in the cardiology-research community due to its potential as a biomarker for screening and risk stratification of acute coronary syndrome (ACS).
Increased choline concentrations have been associated with coronary plaque destabilisation and tissue ischemia, two key components of the biochemical profile in ACS patients that are associated with increased risk of serious cardiac events.
Comprehensive research is now needed to evaluate the use of choline measurements to further understand the underlying pathophysiology of ACS and to assess its clinical utility for detection of ischemia and prediction of adverse cardiac events in suspected cases of ACS.
Further reading
User group meeting for charged aerosol detection
ESA Biosciences has announced the first UK meeting for users and people interested in the theory, applications and use of its multi-award winning Corona CAD HPLC detector.
Ion analysis through HPLC methodologies: webinar
In today's drug development and manufacturing operations, one of the key analysis steps is the measurement of the amount and type of ion or counter ion present in a particular drug formulation
Simultaneous cation and anion measurement by HPLC
ESA Biosciences is promoting a simple and reliable HPLC method for simultaneous measurement of cations and anions, using its Corona Cad universal detector
Fast and accurate assessment of ACS is critical to health outcomes and doctors are looking for more-objective and predictive measures to enhance their clinical decision-making process.
Research in the USA suggests that approximately 25% of all patients visiting emergency rooms complaining of chest pain are suffering from ACS and this excludes patients who were incorrectly diagnosed and discharged from hospital.
In addition to cardiovascular disease, other pressing choline research topics include: its use as a potential therapeutic biomarker and in epidemiological studies to better understand choline as an essential nutrient.
Choline deficiency has been associated with liver damage and its bioavailability is important in brain development of fetuses and newborns.
Choline is also a precursor of acetylcholine, a neurotransmitter involved in muscle control, memory, and many other critical neurological functions.
While choline research has shown significant promise across many clinical and epidemiological applications, the lack of a fast, easy, and readily reproducible way to measure it has made it difficult for scientists to undertake the large-scale studies required to fully examine its potential as a clinical-diagnostic tool or as a nutritional or therapeutic biomarker.
The ESA Choline HPLC Test changes all that, according to the company's vice president of sales and marketing, John Christensen: "Scientists have been asking for an off-the-shelf, validated laboratory assay that can deliver the reliable, reproducible results that this important research demands.
"We designed the Choline HPLC Test for simplicity, specificity, and speed - so researchers can focus precious resources on their study results - not on developing homebrew assays and methods.
"Sample preparation is easy with pre-packaged reagents for precipitation, internal standardisation, and dilution of either whole blood or plasma samples prior to HPLC analysis.
"We obtain specificity through a combination of sample dilution, chromatographic separation, specific enzyme conversion, and selective electrochemical detection.
"The high-throughput test determines choline sample concentration by single-point calibration, enabling analysis time in fewer than 4.5minutes.
"In addition, we validated this assay according to Clinical Laboratory Standards Institute (CLSI) guidelines, so researchers can count on the results assay to assay, experiment to experiment". Request a free brochure from ESA Biosciences ...
• ESA Biosciences: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
