Product category:
Cell/tissue handling and counting
News Release from: Guava Technologies | Subject: CellGrowth
Edited by the Laboratorytalk Editorial
Team on 07 June 2005
Easy and sensitive cell proliferation
analysis
System provides cell proliferation analysis at the single-cell level, offering cell proliferation assessments without the use of more complex fluorescence methods out to five generations
Guava Technologies has introduced a new simple non-radioactive assay designed to improve the performance and sensitivity of cell proliferation assays compared to traditional population-based methods such as 3-Thymidine and MTT (or XTT) The Guava CellGrowth assay, when used in conjunction with the Guava EasyCyte system, provides cell proliferation analysis at the single-cell level, offering cell proliferation assessments without the use of more complex fluorescence methods out to five generations
This article was originally published on Laboratorytalk on 23 Jun 2008 at 8.00am (UK)
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In addition, the assay offers a broader linear range and is more quantitative when compared to colorimetric methods.
The Guava CellGrowth Assay uses a well-characterised cell tracking dye that diffuses freely into cells and is retained within the cell without affecting cellular function.
The dye is not transferred to adjacent cells.
The Guava CellGrowth assay uses two dyes, a cell permanent painting dye and a cell impermanent DNA binding dye, to distinguish live or dead proliferated cells from live or dead resting cells.
For each round of cell division, the relative fluorescence intensity of the dye is decreased by half.
The assay is said to be ideal for monitoring the growth of primary cultures, such as peripheral blood mononuclear cells, activated T or B cells, peripheral blood lymphocytes, thymocytes, hematopoietic cells, splenocytes and fibroblasts.
Used in conjunction with the EasyCyte, the CellGrowth assay provides researchers with a convenient 96-well format for multiple sample analyses increasing productivity and reducing intensive labour costs.
The assay also eliminates the costs and safety issues around the use and disposal of toxic and radioactive chemicals, says Guava.
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