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News Release from: Harvard Apparatus | Subject: Equilibrium Dialyzer
Edited by the Laboratorytalk Editorial
Team on 06 November 2006
Dialysis plates are totally disposable
Harvard Apparatus introduces 96-well Equilibrium Dialyzer for ligand binding experiments including serum protein binding, protein-drug binding, protein-protein binding and protein-DNA binding assays
Harvard Bioscience is introducing the second generation of 96-well equilibrium dialyzer that provides a simple and reliable method to analyze up to 96 samples Equilibrium Dialysis is recognized as the most preferred technique for molecular binding studies as it allows for the direct assay of molecular interactions under normal physiological conditions
This article was originally published on Laboratorytalk on 18 May 2006 at 8.00am (UK)
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50ul, 100ul and 250ul micro-equilibrium dialyzers
These new units have been redesigned for 50ul and 100ul volumes with 4.2x larger membrane surface areas for faster equilibration
Dialyzer now with temperature control
Can be used for studying ligand binding using the method most comparable to in vivo conditions, and studying low affinity interactions which may be undetectable using other methods
Equilibrium Dialysis has also shown itself to be a valuable technique for sample preparation before candidate analysis on a mass spectrometer.
Studies have shown increased target identification when equilibrium dialysis is used before the spectroscopy.
Each well in the plate consists of two chambers separated by a cellulose acetate membrane with a molecular weight cut off of either 5000 or 10,000 Daltons.
Each chamber holds up to 250ul of sample or buffer.
During dialysis the plate needs to be rotated through 360 in a vertical position.
Harvard Bioscience can also supply a one- or two- plate rotator with a clamp attachment for ease of use or an eight plate heated rotator.
The 96-well plate was designed to meet robotic standards of automation, so it can be automated easily with a standard plate footprint and well spacing.
Other equilibrium dialysis products from Harvard Bioscience include the single (double chambered) disposable equilibrium dialyzer for volumes up to 100ml, the re-usable PTFE equilibrium biodialyzer for samples from 25-500ml and the ED2000 for up to 20 samples with volumes from 250ml to 5ml.
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