Product category:
Nucleic acid sequencing and synthesis
News Release from: Human Genetic Signatures | Subject: MethylEasy
Edited by the Laboratorytalk Editorial
Team on 13 April 2004
Trial of DNA bisulphite modification kit
completed
Sydney IVF is studying methylation patterns as part of an overall project aimed at understanding the impact of these patterns on embryonic development
Human Genetic Signatures reports that Sydney IVF has completed a successful trial of its MethylEasy DNA bisulphite modification kit During the trial, Sydney IVF was able to achieve excellent detection of methylated cytosine residues starting from as little as 60 cells - around 400 picograms
This article was originally published on Laboratorytalk on 16 Nov 2006 at 8.00am (UK)
Related stories
Sydney IVF completes successful MethylEasy trial
Sydney IVF completed a successful trial of the MethylEasy DNA bisulphite modification kit from Human Genetic Signatures in 2004
MethylEasy bisulphite kit released for sale
Human Genetic Signatures announced that its first product, the MethylEasy DNA bisulphite modification kit will be released for sale in March 2004
Sydney IVF is studying methylation patterns as part of an overall project aimed at understanding the impact of these patterns on embryonic development.
Changes in DNA methylation have been implicated in aging and disease and have been the focus of rapidly increasing scientific interest in recent years.
Bisulphite conversion is the method currently employed for revealing methylated cytosines in DNA (and was invented by one of the HGS founders, Geoffrey Grigg).
However, conventional conversion techniques can result in the loss of up to 96% of the starting DNA, and are complicated procedures, requiring multiple tube changes and column purification steps.
The MethylEasy DNA bisulphite modification kit reduces bisulphite DNA loss by more than 90%, to give truly representative analysis of methylated cytosine residues.
The Human Genetic Signatures novel technique is a one-tube reaction, requiring no DNA pre-treatment and no column purification.
The technique requires only 100 picograms of starting material.
In-house testing at Human Genetic Signatures, using reliable primers and high quality DNA, has even shown that a signal can be detected with as little as 20 picograms of starting DNA.
These proprietary improvements to the commonly used bisulphite DNA conversion technique are protected by pending US and International patents.
The MethylEasy kit has now been released for sale by Human Genetic Signatures.
In the pipeline, due for release in early 2005, is a high throughput version of the MethylEasy technology, which will be suitable for parallel screening of multiple samples.
• Human Genetic Signatures: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
