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News Release from: Integra Biosciences | Subject: Celline
Edited by the Laboratorytalk Editorial
Team on 22 July 2004
Continuous recombinant protein
production
Protocol uses a novel disposable flask bioreactor to cultivate high densities of eukaryotic cells, minimising handling time and producing significant cost savings
A new applications note is available from Integra Biosciences describing a protocol, developed in conjunction with GSI Creos, using the Celline classic bioreactor for continuous recombinant protein production in baculovirus-infected SF9 cells In recent years, baculovirus expression vectors have become an efficient and popular method for the production of recombinant proteins in insect cell lines
This article was originally published on Laboratorytalk on 26 Jul 2007 at 8.00am (UK)
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Small-scale biomanufacturing process optimisation
In this new field report the authors describe an innovative culture system and protocol that make it possible to optimise simultaneously the three factors of cost, time and quality
Effective small-scale production of antibodies
Using a Celline 350 cell culture system, the Institute for Molecular Medicine and Experimental Immunology (IMMEI) in Germany has transformed the effectiveness of its small-scale antibody production
While recombinant proteins expressed in the cytoplasm of an insect host cell can reach concentrations of up to 1mg/ml, secreted proteins are usually only found at concentrations below 0.01mg/ml.
Obtaining milligram quantities of a secreted protein using a traditional small scale bioreactor or handling large numbers of standard cell culture disposables is consequently time consuming and costly.
The applications study introduces a new protocol, using a novel disposable flask bioreactor, that cultivates high densities of eukaryotic cells, minimises handling time and produces significant cost savings mainly from a 95% reduction of the required serum supplementation.
Further reading
Biomanufacturing benefits from disposable reactors
Technical paper from Integra Biosciences discusses and compares the use of different types of disposable cell culture systems for the manufacturing of biological products in mammalian cells
High-level expression of recombinant proteins
Applications study describes the advantages of a novel disposable flask bioreactor for cultivation of high levels of recombinant proteins in CHO cells
The protocol enables cultivation of SF9 cells in the 20ml cell compartment of the Celline classic bioreactor to a density of up to 1x10e8 cells/ml.
The resultant culture can be continuously maintained for several weeks and cells harvested every sixth day.
Upon transfection of the SF9 cells, with a baculovirus vector, the high cell densities enable production of recombinant protein titres that are typically 50 times higher than that possible with a standard homogeneous cell culture vessel.
In addition because the Celline classic produces concentrated harvesting volumes, laborious concentration of culture supernatants is avoided offering significant savings in the time, labour and material costs of the downstream processing and purification.
Designed for ease-of-use,the Celline classic utilises proprietary membrane technology to separate off the cultivation chamber with an upper semi-permeable membrane through which nutrient can diffuse and a lower one that allows gases to diffuse.
Separate ports allow selective access to the upper nutrient supply chamber and the central cultivation chamber.
This novel compartmentalised arrangement means that medium can be exchanged without influencing the function or growth of the cells and gases.
Consequently, traditional cell growth limitations brought about by a lack of nutrients or the accumulation of metabolic waste can be overcome just as easily as oxygen deficiency.
Such optimised conditions promote significant cell growth and lead to drastically increased cell densities. Request a free brochure from Integra Biosciences ...
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