Product category:
Fermentation and incubation equipment
News Release from: Integra Biosciences | Subject: Celline adhere
Edited by the Laboratorytalk Editorial
Team on 12 July 2005
High-level expression of recombinant
proteins
Applications study describes the advantages of a novel disposable flask bioreactor for cultivation of high levels of recombinant proteins in CHO cells
Integra Biosciences has announced a new applications study, undertaken in conjunction with CEPower (Wadenswil, Switzerland), describing the advantages of a novel disposable flask bioreactor for cultivation of high levels of recombinant proteins in CHO cells A technical poster, describing this work, was short listed from a total of 232 submissions for the poster awards at the recent European Society for Animal Cell Technology (Esact) 2005 meeting in Harrogate, UK
This article was originally published on Laboratorytalk on 19 Mar 2004 at 8.00am (UK)
Related stories
Long-term high-level protein expression
Application study describes the advantages of a novel disposable flask bioreactor for long-term cultivation of high levels of recombinant proteins
Global launch for new disposable flask-bioreactor
Three important international meetings chosen to launch novel disposable flask-bioreactor for long-term, high level protein expression from adherent cells
Optimised for protein expression from adherent cells, the Celline adhere disposable flask bioreactor incorporates a woven polyethylene terephthalate matrix in the cell compartment to provide an ideal surface for attachment of anchorage dependent cells such as CHO, HEK and BHK.
Through use of membrane technology the two-compartment bioreactor Celline adhere enables high cell densities and corresponding product concentrations to be grown in both batch and semi-continuous culture modes.
The study shows that adherent CHO cells were successfully cultivated for several weeks in the CELLine adhere reaching a density of 1.6x10^7 cells/ml.
This represents a ten-fold increase when compared to non-compartmentalised culture systems such as T-Flasks.
As a result of this high cell density a nine-fold improvement in the productivity of the expressed recombinant protein was achieved.
Additional benefits noted by the researchers in using the Celline adhere included decreased volumes of supernatant, lower consumption of serum and lower use of consumables resulting in significant savings in the time, labour and material costs of protein production and downstream processing.
The study demonstrates that the Celline adhere is an ideal tool for rapid and cost effective small scale manufacturing of recombinant proteins in anchorage dependent cells, says Integra. Request a free brochure from Integra Biosciences ...
• Integra Biosciences: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
