Product category:
Genomics
News Release from: Implen | Subject: SlideBooster application note
Edited by the Laboratorytalk Editorial
Team on 20 November 2007
Faster assays through shorter
hybridisation times
New application note demonstrates the outstanding feature of the maintenance-free SlideBooster system: a considerably reduced assay time with consistent or even improved hybridisation quality
Implen's SlideBooster application note describes with amniocentesis, polar body analysis and analysis of tissue sections by Fish three innovative screening methods within the scope of prenatal and preimplantation examination and tumour characterisation The main requirements for the described cytogenetic applications are standardised and highly reproducible experimental conditions with the highest possible sensitivity
This article was originally published on Laboratorytalk on 23 May 2006 at 8.00am (UK)
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Modern hybridisation and washing stations like the SlideBooster Hybridisation Station and the AdvaWash Washing Station (Implen, Germany) allow an automated hybridisation and washing to a sensible degree and guarantee standardised, user independent experimental conditions with minimal variation.
The innovative core of the SlideBooster are integrated micro-agitation chips which generate surface acoustic waves (SAW) to penetrate the hybridisation sandwich and cause quasi-chaotic agitation in the sample volume.
For polar body analysis the duration time of the hybridisation step could be reduced by 50%.
This proves the SlideBooster being an almost mandatory platform for polar body analysis since analysis can be completed within a very narrow time spread.
For Fish on tissue sections the reduction of the hybridisation time by 80% (from 16 hours to three hours) was even more dramatic.
In addition the enhanced binding capacity due to the mixing of nano-volume probes allows a significant reduction of the probe concentration without an influence on the intensity of the obtained signals.
These multiple improvements offer more options for pre- and postnatal genetic applications as well as for tumour cytogenetics and guarantee a feasible and calculable implementation of the Fish analysis in the daily laboratory work.
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