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News Release from: Invitrogen | Subject: Block-iT HiPerform Lentiviral PolII miR RNAi
Edited by the Laboratorytalk Editorial
Team on 14 March 2008
New RNAi vectors launched by Invitrogen
The new vector contains an mRNA stabilising sequence (WPRE) and a nuclear import sequence (cPPT) which generate up to five-fold higher virus titers and emGFP expression than previous versions
Invitrogen has announced the launch of two new vectors for RNAi research The Block-iT HiPerform Lentiviral PolII miR RNAi expression system with emerald green fluorescent protein (EmGFP) offers the expected ability to deliver to nondividing, primary, and hard-to-transfect cells with lentiviral technology coupled to multi-site technology for promoter flexibility making it ideal for difficult applications including in vivo RNAi
The new vector contains an mRNA stabilising sequence (WPRE) and a nuclear import sequence (cPPT) which generate up to five-fold higher virus titers and emGFP expression than previous versions.
The Block-iT Inducible PolII miR RNAi expression system uses a tetracycline-inducible promoter that allows researchers to control miR RNAi expression so that even genes that are essential to the function of a cell may be knocked down.
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