Product category:
Mass spectrometers
News Release from: Lambda Advanced Technology | Subject: Photochem
Edited by the Laboratorytalk Editorial
Team on 23 October 2006
Photochem offers method of measuring
antioxidants.
Photochem from Analytik-Jena, available in the UK from Lambda Advanced Technology, is the first system to be able to quantify both water soluble and lipid soluble antioxidants in a single instrument
Antioxidants are an essential part of the body's defence mechanism against damage to proteins, DNA, lipid components of cell by free radicals such as superoxide, hydroxyl and peroxide Free radical damage can lead to ageing, cancer, asthma, atherosclerosis, cardiac infarction, and arthritis
This article was originally published on Laboratorytalk on 10 Mar 2003 at 8.00am (UK)
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The new Photochem from Analytik-Jena is available in the UK from Lambda Advanced Technology and is the first system to be able to quantify both water soluble and lipid soluble antioxidants in a single instrument.
Photochem uses the principle of photochemiluminescence, combining very fast photochemical radical generation with highly sensitive luminometric detection.
Superoxide anion free radicals are produced in the instrument by optical excitation of a photosensitiser, added in standardised volumes to the sample to be measured.
These are partially removed by reaction with the antioxidants present in the sample.
The remaining radicals cause the detector material, luminol, to luminesce, with the light being measured in a separate cell by a photomultiplier tube.
The anti-oxidative capacity of the sample is quantified by comparison with a calibration curve created using standards of Trolox or ascorbic acid for lipid- and water-soluble antioxidants respectively.
This technique has considerable benefits over the conventional Elisa measurement method, since it generates a free radical for the measurement process that is actually found in nature.
Elisa methods generate 'synthetic' free radicals, whose reaction with antioxidants can be significantly different from 'real' free radicals.
Photochem is ectremely easy to use and offers very high sensitivity.
Sample measurement times are less than three minutes.
There is no complex or time- consuming sample preparation and only a few microlitres of sample are needed.
Sampling, measurement and rinse cycles are performed completely automatically.
Photochem has applications in the food industry, medicine, biology, cosmetics, chemistry, environmental medicine and the pharmaceutical industry. Request a free brochure from Lambda Advanced Technology ...
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