Improve screening for protein crystallisation

Particle sizer can assist both in the screening of appropriate conditions for protein crystallisation and in determining the likelihood of crystals being suitable for structure determination

Malvern Instruments says its Zetasizer Nano S combined static and dynamic light scattering instrument is optimised for the characterisation of proteins in solution prior to crystallisation.

The system is designed for the rapid delivery of accurate and extensive information that can assist both in the screening of appropriate conditions for protein crystallisation and in determining the likelihood of crystals being suitable for structure determination.

Not only does the Zetasizer Nano automatically optimise all instrument settings for each sample,but custom data reports and graphical data presentations make interpretation easier than ever before, says Malvern.

The Zetasizer Nano enables researchers to detect and quantify aggregation, determine the second virial coefficient to find the crystallisation 'sweet spot', and quantify sample polydispersity to increase the likelihood of successful crystallisation.

In addition, it enables users to study the effect of temperature on monodispersity, and offers the ability to automate temperature studies, including melting point and thermal denaturation determinations.

It also allows estimation of prolate and oblate axial ratios and Perrin factor, as well as measurement of hydrodynamic diameter and absolute molecular weight.

Size measurements of proteins as small as 0.6nm and 400Da can be made in their native environments.

As little as 12 microlitres of sample is required, and the sample is recoverable.

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