Metrohm 842 Titrando determines enzyme activity

The Metrohm 842 Titrando's controller has been optimised to determine the activity of an enzyme or the monitoring of the release kinetics of antacid drugs.

The 842 Titrando can also be used to determine the kinetics of acid-base or redox reactions.

Tandem dosing is a new feature offered by the 842 Titrando.

To avoid a break in dosing when the buret is refilled, a second buret takes over the dosing operation without any interruption.

This means that fast reactions and those reactions that have a high reagent consumption can be monitored without any loss of accuracy.

Expanded evaluation criteria allows the user to determine dosing rates and thus the reaction rate over the whole measuring period or within predefined time windows.

Fixed endpoints for the measured quantities - for volume, time and measuring value - are also available.

Tasks performed in the synthesis laboratory differ from those in titration.

Typical synthesis work involves maintaining a constant pH value or performing a multiple dosing operation under strictly defined conditions.

A preset volume has to be added to a solution during a given time span.

During the controlled dosing procedure, the parameters pH (or potential) and temperature are continuously monitored and registered.

This results in a complete documentation of the synthesis sequence.

Dosing can also be carried out continuously in the tandem mode with two Dosinos.

This converts the 842 Titrando into a chemically resistant, high-precision pump.

When a monitored parameter infringes the set limit values, it is up to the user to decide whether to continue the dosing or interrupt and restart it manually or automatically, again applying limit values.

Another feature of the 842 Titrando is its ability to perform control tasks.

External instruments can be controlled by means of freely programmable TTL signals or by communication via an RS-232 interface.

This opens up the possibility of remotely controlling external heating or cooling apparatus, pumps or similar devices.

For dosing, the 842 Titrando uses the Dosino system.

Two Dosinos with their reagent bottles can be accommodated on the Titrando itself, which can control up to four Dosinos.

Besides the enzymatic and the pH Stat titration (Stat), the 842 Titrando offers the following modes: controlled dosing (DOS); endpoint titration (SET); measuring pH, mV and temperature (MEAS); plus additional dosing and liquid handling functions to facilitate sample preparation.

The 842 Titrando comes equipped with one measuring interface; a further measuring interface can be retrofitted.

It is compatible with PC Control 4.0, 840 Touch Control and Tiamo 1.1.

Back to top