Highly sensitive substrates for western blotting

These products need only half to a fifth of the primary and secondary antibodies used by traditional, low-sensitivity substrates, giving decreased antibody consumption and reagent costs

Millipore has announced the availability of two new, high sensitivity chemiluminescent substrates for western blotting applications.

The inexpensive Immobilon Western HRP and AP chemiluminescent substrates provide high signal response at mid-femtogram levels.

Primarily used in Western and dot/slot/spot blotting applications, each substrate has a high signal intensity to facilitate the detection of both high and low abundance proteins.

The Western HRP and AP chemiluminescent substrates use only half to a fifth of the primary and secondary antibodies needed by traditional, low-sensitivity substrates.

As a result, antibody consumption is decreased significantly and reagent costs are reduced.

Available in ready-to-use formats, the two substrates are compatible not only with polyvinylidene fluoride (PVDF) and nitrocellulose membranes, but also commonly used buffers and blocking reagents.

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