Purified Mouse Apo A1 used in CETP study

Edited by the Laboratorytalk editorial team May 18, 2009

The role of cholesteryl ester transfer protein (CETP) in the development of atherosclerosis was the objective of a 2006 publication in Arteriosclerosis, Thrombosis, and Vascular Biology.

The publication is entitled 'Cholesteryl Ester Transfer Protein Decreases High-Density Lipoprotein and Severely Aggravates Atherosclerosis in APOE*3-Leiden Mice' by Marit Westerterp, Caroline C van der Hoogt, Willeke de Haan, Erik H Offerman, Geesje M Dallinga-Thie, J Wouter Jukema, Louis M Havekes and Patrick CN Rensen.

In the publication, the authors evaluated the effect of human CETP expression on atherosclerosis in APOE*3-Leiden Mice with a humanised lipoprotein profile.

Plasma Apo AI concentrations were determined using a sandwich enzyme-linked immunosorbent assay (ELISA) with Meridian Life Science (Biodesign International) Purified Mouse Apo AI as a standard.

Marit Westerterp et al concluded that CETP expression in E3L mice shifts the distribution of cholesterol from HDL to VLDL/LDL, reduces plasma-mediated SR-BI-dependent cholesterol efflux, and represents a clear pro-atherogenic factor in E3L mice.