HPLC columns for synthetic oligonucleotides

Phenomenex introduces Clarity Oligo-RP columns for the reversed phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity

Ideal for trityl-off purification of DNA, RNA and modified or labeled oligos, the new columns easily separate full-length oligos from N-1 and other failed sequences.

The specially designed Oligo-RP media, based on Phenomenex's proprietary Twin (two in one) composite particle technology, recognises minute differences in interaction features of two oligos, providing better resolution than other methods.

Oligo-RP columns perform at 90% or higher purity levels with typical recovery rates of 70%.

The Oligo-RP phase allows high loadability and delivers high recovery and purity while eliminating the need for an extra desalting step.

"The demand for high-purity oligonucleotides is growing rapidly," commented Cathy Cordova, brand manager for Phenomenex.

"This efficient, high-capacity HPLC solution resolves failed N-1 sequences better than other offerings, producing a higher level of purity".

The new Oligo-RP columns are part of Phenomenex's Clarity BioSolutions portfolio of products for synthetic oligonucleotide purification.

From low purity/low loading to high purity/high loading tools, Phenomenex offers a range of solutions along with hands-on technical and applications support.

Clarity Oligo-RP columns have loading capacities from 50 nmole to 50 umole and are offered in sizes for analytical, semi-preparative and preparative applications.

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