Product category:
Proteomics
News Release from: Perkin Elmer LAS (UK) | Subject: Protein purification platform
Edited by the Laboratorytalk Editorial
Team on 16 August 2002
Automated solution for protein
purification
Collaboration has resulted in a 'breakthrough' platform for automating labour-intensive steps involved in protein purification
Novagen, a provider of advanced reagents for molecular biology and proteomics research, and PerkinElmer, a provider of drug discovery, life science research and genetic disease screening solutions, have announced an agreement to co-market a novel new solution for high throughput, automated recombinant protein purification "Extending our range of powerful liquid handling technologies to streamline the developing science of proteomics is a major goal for PerkinElmer Life Sciences," said Warren Bock, vice president and general manager for liquid handling products
This article was originally published on Laboratorytalk on 19 Apr 2002 at 8.00am (UK)
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"We are first to market in this area because our systems are uniquely suited to integrate a broad range of products in all facets of drug discovery research." "Novagen remains committed to offering only the highest quality reagents and kits, such as our RoboPop purification kits, which serve the emerging proteomics market," said Robert Mierendorf, president of Novagen and chief technology officer of CN Biosciences.
"Our greatest satisfaction derives from providing tools that enable new discoveries." The collaboration between PerkinElmer and Novagen has resulted in a breakthrough platform for automating labour-intensive steps involved in protein purification.
Conventional purification methods are time consuming and labour intensive, requiring centrifugation and mechanical steps for cell harvest and lysis prior to purification.
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Using PerkinElmer's II EX robotic liquid handler and Novagen's RoboPop protein purification kits, the platform performs 96-well format purification of His-Tag and GST-Tag fusion proteins directly from E coli cultures without mechanical lysis or centrifugation.
Novagen's RoboPop system is uniquely suited for automated protein purification because it eliminates the need for centrifugation and enables the entire process from cell growth through purification to be performed in a single deep well plate.
The kits feature PopCulture reagent, rLysozyme solution, and Benzonase nuclease for efficient extraction of intact proteins from total cultures, plus His-Mag or GST-Mag agarose beads and corresponding buffers for high capacity magnetic affinity purification.
The MultiProbe II EX system, when integrated with PerkinElmer's Integration Platform, allows for complete walk-away automation of the magnetic affinity purification protocol.
The system's expanded deck configuration accommodates the RoboPop culture plate, reagents and Magnetight HT96 separation stand.
The RoboPop kits include everything needed to achieve the entire process, from cell culture of 96 recombinant clones to purified protein in an HTP 96-well format, including reagents and plasticware.
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