Product category:
Polymerase chain reaction (PCR)
News Release from: Promega UK | Subject: ImProm-II
Edited by the Laboratorytalk Editorial
Team on 23 October 2002
Robust synthesis of first strand cDNA
Reverse transcriptase is optimised for the efficient and robust synthesis of first-strand cDNA from RNA for further amplification
Ideal for all molecular biologists performing reverse transcription (RT) and RT-PCR, the ImProm-II reverse transcriptase is optimised for the efficient and robust synthesis of first-strand cDNA from RNA for further amplification The ImProm-II enzyme can be used to reverse transcribe RNA templates starting with either total RNA or poly(A) +mRNA
This article was originally published on Laboratorytalk on 4 Sep 2002 at 8.00am (UK)
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A fully optimised reaction buffer coupled with a powerful reverse transcriptase enzyme makes it possible to carry out reliable full-length cDNA synthesis for the reproducible analysis of rare or long messages.
Versatile and efficient, ImProm-II can be used to reverse transcribe RNA templates of over 8.5 kilobases and can be used to incorporate standard and amino-allyl modified nucleotides, typically used in the preparation of Cy3 and Cy5 labelled fluorescent probes.
cDNA synthesis conditions have been formulated for stand-alone applications or for the easy transition to gene-specific target amplification.
Fully scaleable, volumes from 1µl to 10µl of the reverse transcription reaction can be directly amplified using Promega Taq DNA Polymerase in coupled or uncoupled PCR reactions.
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