Product category:
Polymerase chain reaction (PCR)
News Release from: Promega UK | Subject: Sistrike and Silentgene-2
Edited by the Laboratorytalk Editorial
Team on 12 August 2004
Hairpin cloning systems for RNAi studies
Improved signal and increased convenience compared to synthetic siRNA are claimed for these two hairpin cloning systems
Promega has expanded its range of products for RNA interference studies with two new hairpin cloning systems The new products, Sistrike U6 hairpin cloning system-hmGFP and Silentgene-2 U6 hairpin cloning systems (human), offer improvements in selection and screening compared to synthetic siRNA
This article was originally published on Laboratorytalk on 4 Sep 2002 at 8.00am (UK)
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The Sistrike U6 hairpin cloning system-hmGFP is a short hairpin RNA expression vector containing an internal fluorescent marker.
This marker is an improved synthetic version of the green fluorescent protein (GFP) which has greater fluorescence and less cytotoxicity than the native gene.
The increase in signal permits easier determination of transfection efficiency and subsequent selection by fluorescence-activated cell sorting (Facs), ultimately leading to improved RNAi results, says Promega.
The Silentgene-2 U6 hairpin cloning systems (human) allow rapid screening of target RNAi sequences through a PCR cassette-based vector system.
This approach requires just a single amplification step to produce a U6 promoter driven PCR cassette that can be directly transfected into cells for rapid screening.
Once the best target has been identified, the PCR product can be sub-cloned directly into the pre-digested, dephosphorylated vector for long-term experiments.
A choice of antibiotic selection marker (none, neomycin, hygromycin or puromycin) provides considerable flexibility for the researcher.
Subsequent identification of positive recombinants can easily be achieved following an EcoRV digestion with blue/white screening.
The Silentgene systems are the only products with this option, says the company.
These new systems complement Promega's existing broad range of RNAi products including vectors, expression systems and reagents.
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