Product category:
Proteomics
News Release from: Qiagen | Subject: Two-Step
Edited by the Laboratorytalk Editorial
Team on 18 August 2003
Two-step affinity purification system
Complete standardised solution for the purification of ultrapure full-length recombinant proteins, even from eukaryotic cell lysates
The Two-Step affinity purification system provides a complete, standardised solution for the purification of ultrapure (>98% pure) full-length recombinant proteins, even from eukaryotic cell lysates Ultrahigh purity is achieved by sequential affinity purification via two small affinity tags, the 6xHis and Strep-tag
This article was originally published on Laboratorytalk on 6 May 2005 at 8.00am (UK)
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The complete system includes expression vectors, purification matrices, and antibodies.
HisStrep pQE-TriSystem vectors allow expression of proteins carrying a 6xHis and Strep-tag in E coli, insect, and mammalian cells using a single construct.
Once expressed, the presence of a 6xHis and Strep-tag enables purification of ultrapure, full-length proteins by sequential affinity purification on Ni-NTA and Strep-Tactin matrices.
Purification can be performed on a micro-scale using Ni-NTA- or Strep-Tactin-coated magnetic beads and on larger scales using Ni-NTA or Strep-Tactin Superflow.
Highly specific mouse monoclonal antibodies are available for sensitive detection of 6xHis-tag or Strep-tag epitopes.
The Two-Step affinity purification system provides standardised purification of virtually any protein with extremely high purity, eliminating the need to develop protein-specific purification protocols and the attendant costs in time and materials.
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