Product category:
Proteomics
News Release from: Qiagen | Subject: Qproteome
Edited by the Laboratorytalk Editorial
Team on 04 February 2005
Making the proteome more manageable
Kits enable fast, standardised fractionation of complex protein matrices for initial purification or preprocessing for Page or mass spectroscopic analysis
A major problem facing protein researchers is the sheer complexity of the proteome Purification and analysis of low-abundance species is especially challenging
This article was originally published on Laboratorytalk on 26 Apr 2006 at 8.00am (UK)
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To address these problems, Qiagen has used its expertise in sample preparation to develop the Qproteome range of kits.
The kits, which are based on spin or gravity-flow columns, enable fast, standardised fractionation of complex protein matrices for initial purification or preprocessing for Page or mass spectroscopic analysis.
These kits can also be used to localise or characterise biomarkers in cells grown under different conditions or isolated from different tissues.
Easy-to-use kits are available that separate proteins on the basis of glycosylation patterns, their subcellular localisation, their affinity to nucleic acids, and their overall solubility.
An additional kit is available for depletion of albumin and IgG from serum or plasma samples.
This depletion facilitates analysis of low-abundance blood proteins, whose presence may otherwise be obscured by the large proportion of albumin and IgG in the sample.
Separation is fast, efficient, and reproducible and delivers active protein into distinct fractions, ready for further downstream analysis.
The wide choice of kits - each of which separates proteins using a different physical principle - ensures that researchers can find a kit that offers effective and reproducible reduction in complexity of their proteomics samples, says Qiagen.
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