Product category:
Polymerase chain reaction (PCR)
News Release from: Qiagen | Subject: FastLane cell cDNA kit
Edited by the Laboratorytalk Editorial
Team on 06 May 2005
First-strand cDNA in 45 minutes
High-speed preparation of cDNA from cultured cells without RNA purification makes it easy to perform real-time, two-step RT-PCR analysis of several samples within a few hours
With the FastLane cell cDNA kit, only 45 minutes are required to prepare first-strand cDNA directly from cultured cells - no RNA purification steps are necessary The high-speed procedure makes it easy to perform real-time, two-step RT-PCR analysis of several samples within a few hours
This article was originally published on Laboratorytalk on 20 Aug 2001 at 8.00am (UK)
Related stories
Bulk recombinant enzymes
The efficient and highly specific removal of His tags offered by the TagZyme system makes it the ideal choice for the generation of proteins free from vector-encoded amino acids
The FastLane cell cDNA kit integrates rapid cell lysis, immediate RNA stabilisation, elimination of genomic DNA, and efficient and sensitive reverse transcription, says Qiagen.
The simple workflow makes the kit ideal for applications such as validation of siRNA-mediated gene knockdown or snapshot analysis of gene expression in cells under different conditions.
Highly reproducible and sensitive reverse transcription can be achieved, says the company.
Rapid cell lysis with simultaneous RNA stabilisation ensures RNA transcripts remain intact, while optimal reaction conditions for reverse transcription provide high cDNA yields.
Even difficult templates, such as those with high GC-content or complex secondary structure, are successfully reverse-transcribed.
To ensure only RNA is detected in real-time RT-PCR, the FastLane cell cDNA kit uses a novel buffer to effectively eliminate genomic DNA contamination.
Time and effort are saved as there is no need to design RNA-specific primers or probes, which may not even be possible with some genes.
• Qiagen: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
