Product category:
Polymerase chain reaction (PCR)
News Release from: Qiagen | Subject: QuantiFast kits
Edited by the Laboratorytalk Editorial
Team on 24 January 2007
QuantiFast kits - fast PCR on any
real-time cycler
QuantiFast Kits are ready-to-use master mixes that eliminate the need for optimisation of reaction and cycling conditions; simply follow the handbook protocol to get fast and reliable results
QuantiFast Kits deliver significantly faster results in real-time PCR With time savings of up to 60% on existing cyclers, there is no need for costly hardware upgrades
This article was originally published on Laboratorytalk on 20 Aug 2001 at 8.00am (UK)
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In addition, no PCR optimisation is required, even if using previously established assays.
Kits are available for SYBR Green or probe-based detection in PCR, two-step RT-PCR, and one-step RT-PCR.
A specially developed fast PCR buffer containing the patent-pending additive Q-Bond greatly reduces denaturation, annealing, and extension times.
The buffer also contains HotStarTaq Plus DNA polymerase, which requires only three or five minutes at 95C for activation.
For one-step RT-PCR, the optimised QuantiFast RT Mix enables cDNA synthesis in just ten minutes.
Accurate quantification of even low copy numbers are achieved with QuantiFast Kits.
A balanced combination of KCl and NH4Cl in the fast PCR buffer promotes specific primer annealing, enabling high PCR specificity and sensitivity.
In addition, HotStarTaq Plus DNA polymerase provides a stringent hot start, preventing the formation of nonspecific products.
QuantiFast Kits are ready-to-use master mixes that eliminate the need for optimisation of reaction and cycling conditions.
Simply follow the handbook protocol to get fast and reliable results.
The kits are compatible with cyclers that use ROX dye for fluorescence normalisation (no optimisation of Rox concentration is necessary) and with cyclers that do not.
Novel Q-Bond technology is also used by the Qiagen Fast Cycling PCR kit, which is designed for standard and challenging end-point PCR applications.
The kit provides fast and specific PCR on standard as well as fast-ramping thermal cyclers.
Significant time savings of up to 75% can be achieved.
QuantiFast Kits and the Qiagen Fast Cycling PCR kit are intended for research use.
Qiagen adds that no claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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