Product category:
Polymerase chain reaction (PCR)
News Release from: Qiagen | Subject: QuantiFast Probe RT-PCR Kits
Edited by the Laboratorytalk Editorial
Team on 04 October 2007
Faster one-step RT-PCR on any real-time
cycler
QuantiFast Probe RT-PCR Kits deliver significantly faster results in real-time one-step RT-PCR with sequence-specific probes, such as TaqMan probes
The kits are a new addition to the range of QuantiFast Kits, which provide fast cycling on any real-time cycler With time savings of up to 60% on existing cyclers, there is no need for costly hardware upgrades
This article was originally published on Laboratorytalk on 20 Aug 2001 at 8.00am (UK)
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A specially developed fast RT-PCR buffer containing the patent-pending additive Q-Bond greatly reduces denaturation, annealing, and extension times.
The buffer also contains HotStarTaq Plus DNA Polymerase, which requires only five minutes at 95C for activation.
The optimized QuantiFast RT Mix enables cDNA synthesis in just ten minutes.
Accurate quantification of even low copy numbers is achieved with the kits.
A balanced combination of KCl and NH4Cl in the fast RT-PCR buffer promotes specific primer annealing, enabling high PCR specificity and sensitivity.
In addition, HotStarTaq Plus DNA Polymerase provides a stringent hot start, preventing the formation of nonspecific products.
QuantiFast Probe RT-PCR Kits are ready-to-use master mixes that eliminate the need for PCR optimisation.
Simply follow the handbook protocol to get fast and reliable results.
The kits are compatible with cyclers that use ROX dye for fluorescence normalisation (no optimisation of ROX concentration is necessary) and with cyclers that do not.
QuantiFast Kits are intended for research use.
Qiagen says no claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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