Product category:
Chromatography accessories
News Release from: Selerity Technologies | Subject: Technical Note 804
Edited by the Laboratorytalk Editorial
Team on 10 October 2003
HTLC columns take the heat
Technical note describes benefits of newly-developed polydentate silica specifically configured to remedy deficiencies and minimise column degradation
High temperature liquid chromatography (HTLC) specialist Selerity Technologies describes, in a new free technical note, the requirements for silica columns to withstand high temperatures Selerity has developed a polydentate silica phase specifically configured for HTLC to remedy previous deficiencies and minimise column degradation
This article was originally published on Laboratorytalk on 29 Apr 2003 at 8.00am (UK)
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The reduced viscosities experienced at elevated temperatures result in a significantly lower backpressure, allowing for an increased flow rate, which reduces analysis time.
Higher column efficiencies due to better diffusion rates can improve resolution and sensitivity.
Traditionally, silica columns employed in high performance liquid chromatography (HPLC) have been unstable at temperatures above 60C under reverse phase conditions.
Silica columns have a propensity to degrade as water attacks the underlying support particles.
With polydentate polymer protecting the silica surface, Selerity's Blaze columns safeguard silica particles against hydrolytic breakdown providing an efficient and stable column up to 100C.
The Selerity Blaze polydentate phase protects the silanol groups against attacks.
The polymer attaches to the silica structure at a number of points forming a protective cap over the hydrolytically unstable siloxane groups.
Blaze columns are available in a variety of lengths and sizes and benefit from high temperature, low dead volume housing.
The technical note further discusses the benefits of polydentate chemistry, including hydrolytic breakdown mechanisms, and provides information on other columns that Selerity has tested and found suitable for HTLC.
It confirms that high temperature analyses have proven to be successful on polymer, carbon and zirconia columns with stainless steel housing and stainless steel end fittings.
The technical note (number 804) is available free of charge; simply email Selerity.
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