Product category:
Antibodies
News Release from: Serotec | Subject: Alexa Fluor
Edited by the Laboratorytalk Editorial
Team on 22 March 2005
Antibodies have brighter more stable
fluorescence
Brighter and more stable than comparable conjugates, and insensitive to changes in pH over a broad range making them useful for multicolour flow cytometry and fluorescence/confocal microscopy
Serotec has entered into license agreements with Molecular Probes to label many of its primary antibody range with Alexa Fluor 405, 488 and 647 dyes These fluorochromes are said to exhibit brighter and more stable fluorescence than comparable conjugates, and are insensitive to changes in pH over a broad range, making them more versatile for multicolour flow cytometry and fluorescence/confocal microscopy
This article was originally published on Laboratorytalk on 18 Mar 2005 at 8.00am (UK)
Related stories
Fast results from guinea pig allergy kit
Elisa kits quantify total and ovalbumin-specific immunoglobulin E in just 2.5 hours, compared to the one week typical of some other tests
Serotec's current range includes antibodies to mouse, rat and human CDs and to macrophage markers together with their associated controls.
Serotec also offers custom conjugation of clients' antibodies to these dyes.
Alexa Fluor 488 is described as the best green fluorophore available and outperforms conjugates of fluorescein and Cy2 at every level.
The newer Alexa Fluor 405 is the optimal dye for the blue (400-410nm) excitation range and can be used with violet and krypton lasers.
It has minimal overlap with green conjugates making it ideal for multicolour applications.
Designed as an alternative to APC and Cy5, Alexa Fluor 647 produces similar spectra but has all the benefits of Alexa Fluor range.
• Serotec: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
