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Chromatography accessories
News Release from: SeQuant | Subject: Glucosinolates
Edited by the Laboratorytalk Editorial
Team on 21 June 2007
Glucosinolates can be separated by
Zic-Hilic
In a typical separation, glucoraphanin and glucoiberin (two glucosinolates found in broccoli) were separated in ten minutes at a flow rate of 0.5ml/min using Zic-Hilic chromatographic media
Glucosinolates are anionic secondary metabolites which are phytochemicals found in cruciferous vegetables (eg, broccoli) and are of interest due to their role in the prevention of cancer These compounds can be readily separated using Zic-Hilic hydrophilic interaction chromatographic media
This article was originally published on Laboratorytalk on 12 Dec 2005 at 8.00am (UK)
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Tips for extraction of hydrophilic biomolecules
Pipette tips contain the unique 'zwitterionic' Zic-hilic chromatographic particle for extraction and purification of hydrophilic biomolecules such as glycopeptides.
A practical guide to Hilic
Hydrophilic interaction liquid chromatography (Hilic) is an extremely useful technique for the separation of polar and hydrophilic compounds via HPLC; SeQuant has prepared a tutorial guide
An analytical method for the separation has recently been reported that employs an ammonium formate buffer (pH 4.5) with acetonitrile to provide baseline separation with a short separation time ['Improved hydrophilic chromatography method for the identification and quantitation of glucosinolates' by KL Wade, IJ Garrard and JW Fahey, J Chromatogr, in press].
This analytical method has a number of significant benefits compared to previously reported methods for the separation of glycosinolates.
One popular method employs ion-pairing reagents, which are not desirable as a desalting step is required if mass spectroscopic detection is required (as is frequently the case).
Other commonly used methods for the separation involve enzymatic desulphation followed by separation on a C18 column.
While this method is successful, it suffers from the disadvantage that the separated compounds are no longer biologically active nor can they be converted to isothiocyanates or other biologically active compounds.
The method based on Zic-Hilic is reported to be "much more robust and flexible, giving better separation of a greater range of glucosinolates, with a much longer column life". Request a free brochure from SeQuant ...
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