Product category:
Chemicals and biochemicals
News Release from: Sigma-Aldrich
Edited by the Laboratorytalk Editorial
Team on 29 July 2005
High quality analysis of larger
oligonucleotides
Sigma-Genosys has incorporated into its quality control (QC) lab an LC-MS system from Novatia to improve molecular weight determination of synthetic oligonucleotides and peptides
The new LC-MS system from Novatia improves the molecular weight determination of larger synthetic oligonucleotides (>50 bases) as well as synthetic peptides Novatia specialises in developing an integrated system encompassing hardware, software, method development and automated data analysis
This article was originally published on Laboratorytalk on 1 Mar 2004 at 8.00am (UK)
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Traditionally, larger synthetic oligonucleotides are analysed by capillary electrophoresis and by Page analysis.
When molecular weight information is needed, Maldi-Tof mass analysis is carried out.
Traditional Maldi-Tof MS analysis, while rapid (<5 seconds per sample) and relatively simple, provides inadequate mass spectra data if any, for larger oligonucleotides.
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This is due primarily to poor ionisation of the large oligonucleotides.
With the integrated Novatia LC-MS system, Sigma-Genosys is able to obtain high quality mass analysis of larger oligonucleotides.
Traditional LC-MS techniques can be time consuming and not amenable to high throughput analysis.
The LC-MS system by Novatia along with the methods and automated post-analysis software provides Sigma-Genosys with a high throughput (one sample per minute) LC-MS technique for the analyses of larger oligonucleotides.
The well-integrated instrument and protocols developed by Novatia reduced the IOQ (installation/operating qualification) time to days from the weeks normally associated with high-technology analytical systems.
The Novatia system also provides Sigma-Genosys with the ability to obtain higher quality mass spectra data in a high throughput format.
The improved resolution obtained by LC-MS provides more accurate molecular weight of the larger oligonucleotides.
This allows detection of base substitutions as well as being able to determine the presence and identification of potential contaminates and base deletions.
Sigma-Genosys has continued to examine and implement cutting edge technologies for continued improvement in product quality.
The addition of the Novatia LC-MS system ensures that customers are provided with the highest possible quality oligonucleotides and peptides, it says.
Sigma-Aldrich's biochemical and organic chemical products and kits are used in scientific and genomic research, biotechnology, pharmaceutical development, the diagnosis of disease and as key components in pharmaceutical and other high technology manufacturing.
It has customers in life science companies, university and government institutions, hospitals, and in industry.
Over one million scientists and technologists use its products.
Sigma-Aldrich operates in 35 countries and has over 6800 employees.
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