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Product category: Nucleic acid sequencing and synthesis
News Release from: Sigma-Aldrich | Subject: SpyLine Poly A+
Edited by the Laboratorytalk Editorial Team on 23 January 2006

Sigma-Aldrich launches SpyLine Poly A+
capture kit

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SpyLine is the newest addition to Sigma's RNA product offerings and is an excellent tool for rapidly identifying the knockdown of gene expression for functional profiling of biological processes

Sigma-Aldrich introduces the SpyLine Poly A+ capture kit (product codes: SPY1 and SPY4) "The SpyLine Poly A+ Capture Kit simplifies knockdown measurements and gene expression analysis by allowing capture and amplification of mRNA in a single plate," said Keith Jolliff, strategic marketing director at Sigma-Aldrich

"We are very excited about the sensitivity and reproducibility of the kit, making it an excellent addition to our RNA product offering".

The kit features the SpyLine Poly A+ Capture Plate, a 96-well PCR plate coated with oligo(dT) for selective capture of poly A+ mRNA from crude mammalian cell lysates.

Reagents can be added directly to this capture plate for subsequent RT-PCR or real-time quantitative RT-PCR cycling and analysis.

In addition, the 96-well format is suitable for automation.

High throughput protocols are available for the Biomek FX Workstation.

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