Product category:
Chemicals and biochemicals
News Release from: Sigma-Aldrich | Subject: Mission TRC
Edited by the Laboratorytalk Editorial
Team on 23 January 2006
Expanding shRNA libraries of the RNAi
Consortium
Sigma-Aldrich announces the newest release of the Mission TRC shRNA human and mouse libraries for RNA interference (RNAi)-based research
The Mission TRC shRNA human and mouse libraries for RNA interference (RNAi)-based research now carry approximately 65,000 shRNA vector constructs that target 9500 human genes and 3400 mouse genes The newly added libraries include extensive coverage of the druggable genome and other highly studied targets
This article was originally published on Laboratorytalk on 6 Feb 2006 at 8.00am (UK)
Related stories
Launch of first lentiviral shRNA gene family set
Sigma-Aldrich, a member of the RNAi Consortium (TRC), introduces the first available shRNA gene family set in lentiviral format for direct use in gene silencing.
RNAi product line grows further
When completed, these shRNA clone libraries will comprise a comprehensive collection of 150,000 pre-cloned lentiviral-based shRNA vector constructs targeting 15,000 human genes and 15,000 mouse genes
Each clone is available from Sigma-Aldrich in bacterial glycerol stock, ready-to-transfect purified plasmid DNA, and lentiviral particles for direct transduction.
Sigma-Aldrich is the exclusive source of the TRC shRNA libraries in the plasmid DNA and lentiviral formats.
The lentiviral-based shRNA libraries provide researchers with tools for studying gene function and disease in standard cell lines and recognised difficult cell types, such as primary, non-dividing, growth-arrested or terminally differentiated cells.
Such cell lines are typically not amenable to standard transfection or genomic integration by other viral delivery systems, but they may be transduced efficiently with lentiviral particles.
Harvard University and The Broad Institute of the Massachusetts Institute of Technology and Design are leading the design and development of the TRC libraries, which will be expanded on a quarterly basis to include new gene targets.
Eventually, the libraries will comprise a comprehensive collection of 150,000 pre-cloned lentiviral-based shRNA vector constructs targeting 15,000 human genes (Mission TRC-Hs1.0) and 15,000 mouse genes (Mission TRC-Mm1.0).
• Sigma-Aldrich: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
