New nitrocellulose microplate suits immunology
A
Whatman International product story
Edited by the Laboratorytalk editorial team
Aug 21, 2007
Whatman is offering a new nitrocellulose microplate technology that addresses immunology applications
The new Whatman ELISA Unifilter offers speed, sensitivity and simple washing protocols with nitrocellulose filter plates.
ELISA performed with the Whatman ELISA Unifilter takes less time than traditional methods using regular microplates.
The ELISA filterplate allows researchers to utilise the excellent protein-binding characteristics of nitrocellulose in a standard well format.
Coating the nitrocellulose filter with antibodies takes only minutes, compared with overnight procedures employed for coating polystyrene microplates.
Also, the use of vacuum filtration greatly reduces the time required and enables quantitative collection of filtrate into a collection plate.
Whatman utilises a the company's patented process to encapsulate the filter media, which ensures no cross talk or contamination between wells.
The propriety technology allows for a large number of applications, from sample preparation, genomics and filter based assays to filtration technology, sample clean-up and isolation of nucleic acids.
The new technology is also suitable for other types of ligand binding applications such as hybridoma supernatant screening.
Solutions are easily vacuumed to waste using a vacuum manifold.
Chemiluminescent assays can be read in the plate.
If the ELISA product is eluted then centrifugation is preferred.
The microplate is compatible with automated systems.
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