Product category:
Nucleic acid sequencing and synthesis
News Release from: Syngene | Subject: G:Box Chemi
Edited by the Laboratorytalk Editorial
Team on 25 April 2006
Differential protein expression: one
Western blot
New fluorescence application of G:Box Chemi makes studying differential protein expression on one western blot a reality
Syngene reports that its G:Box Chemi multi-purpose imaging system is being used by top French reagent supplier, Ozyme, as part of a unique fluorescence based method of rapidly detecting differential protein expression on single Western blots Using fluorescent Qdot technology, scientists at Ozyme have developed a simple protocol for differentially labelling proteins on Western blots known as FluoWest
This article was originally published on Laboratorytalk on 2 Mar 2006 at 8.00am (UK)
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They are then using a G:Box Chemi system to generate images of FluoWest blots showing different proteins labelled with multiple fluorescent colours on one blot.
Olivier Varet, product manager at Ozyme explained: "Multiplex analysis of proteins can be used to determine up and down regulation of specific proteins, to diagnose a pre-disposition to a specific disease or to get an accurate quantification".
"However, many current methods of labelling Westerns are unsuitable for this application because they only produce one colour or a white chemiluminescent emission, which means that scientists have to generate a series of blots to compare protein expression".
"Using FluoWest, scientists can save time by analysing a number of different proteins from the same blot".
"This is especially useful when these proteins have the same molecular weight or are very close together." "We decided to use the G:Box Chemi to analyse our FluoWest blots because it is one of the few CCD analysers that has the right combination of camera, Epi-UV lighting and filters which are so essential for successfully generating high quality images of this type of blot," continued Varet.
Laura Sullivan, Syngene's divisional manager stated: "This is a very exciting and original application of our new G:Box Chemi system, offering quick and simple analysis of multiple different proteins on single Western blots". Request a free brochure from Syngene ...
"For researchers wanting to increase the throughput of Western blot analysis to detect differential protein expression, using FluoWest with a G:Box Chemi is an excellent choice.".
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