Product category:
Nucleic acid sequencing and synthesis
News Release from: Syngene | Subject: Dyversity
Edited by the Laboratorytalk Editorial
Team on 14 September 2006
Accurate detection of nanogram amounts
of protein
Syngene, a manufacturer of image analysis solutions, reports that Dyversity, its multi-functional imager, can now rapidly detect 0.5ng of Sypro Ruby stained protein in less than a second
This new application of Dyversity makes it an ideal system for high-throughput gel-based proteomics Syngene's technical team used a Dyversity system with a Cy dye lighting module, a dual wavelength transilluminator, a blue light converter, UV, long pass, Cy3 dye and SG emission filters to image 1D acrylamide gels stained with Sypro Ruby, containing 1000-0.1ng of molecular weight standard PeppermintStick (Invitrogen)
This article was originally published on Laboratorytalk on 16 Feb 2006 at 8.00am (UK)
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CCD is alternative to laser scanner for 2D gels
Syngene announces the availability of Dyversity, its new automated imager, designed to save time with producing precise images of 2D protein gels
CCD is ten times faster than laser for 2D gel
Syngene reports a new study which demonstrates that Dyversity, its automated 2D gel imager, can capture high quality 2D protein gel images significantly faster than the leading laser scanner
The gels were imaged under four conditions: Cy2 excitation with a Cy3 emission filter; Cy2 excitation with a UV emission filter; medium-wave UV excitation with a UV filter and medium-wave UV excitation with a blue light converter and SG filter.
The results showed using the medium-wave UV excitation with a blue light converter and SG filter, Dyversity could detect 5ng of Sypro Ruby stained protein in four seconds.
The Cy2 illumination with either Cy3 or long pass emission filter allowed detection of 0.5ng of protein in two seconds, but the best results were obtained using the medium-wave UV and a UV filter which detected 0.5ng of protein, in under one second, unrivalled imaging performance for a CDD-based analyser.
Dyversity can acquire images of small amounts of fluorescently labelled protein so rapidly because it has a 90 micron resolution, 16-bit CCD camera.
This provides Dyversity with the fastest capture time per channel for Cy dyes of any CCD system on the market today, and means it is an excellent alternative to a laser-based scanner.
Laura Sullivan, Syngene's divisional manager explained: "The advantage of using Dyversity is that you can change the imaging conditions to generate the exceptional results you would normally obtain from an expensive laser scanner". Request a free brochure from Syngene ...
"Any scientist wanting to accurately detect small quantities of proteins in 1D or 2D gels should look at Dyversity, as it is currently the fastest and most flexible imager for this application.".
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