Detection of proteins stained with Flamingo dye
A
Syngene product story
Edited by the Laboratorytalk editorial team
Sep 29, 2006
Dyversity can now accurately and rapidly detect protein stained with Flamingo, the newer protein dye from Bio-Rad, making Dyversity a cost-effective alternative to laser scanning
Syngene's technical team used Dyversity, which consists of a light tight darkroom containing a 16-bit CCD camera fitted with a Cy dye lighting module, dual wavelength transilluminator, UV, long pass and Cy3 dye emission filters, to image 1D acrylamide gels.
These gels, containing 1000-0.1ng of molecular weight standard PeppermintStick (Invitrogen) stained with Flamingo, were imaged under three different conditions: Cy2 excitation with Cy3 emission filter; Cy2 excitation with UV emission filter and medium-wave UV excitation with a short pass emission filter.
Syngene's technical experts saw Dyversity could image 5ng of Flamingo stained protein in two seconds using medium-wave UV excitation and short pass emission filter.
However, using Cy2 illumination with the Cy3 or UV emission filter, Dyversity was ten times more sensitive, detecting as little as 0.5ng of protein in the same time.
Dyversity can quickly image such minute amounts of Flamingo stained protein because its high quality camera has the fastest capture times per channel for fluorescent and visible dyes of any current CCD based system.
Dyversity's darkroom also has a large door opening to accommodate a range of gel sizes and users can easily add new filters and lighting as they need to, making this the most versatile image analyser available.
Laura Sullivan, Syngene's divisional manager commented: "We are pleased Dyversity can image one of the newer protein dyes with such speed and sensitivity.
"This unrivalled performance coupled with the flexibility to upgrade the system as different protein dyes are commercially launched, means Dyversity offers an affordable and precise alternative to laser-based scanners for generating perfect images of 1D or 2D protein gels.".
Not what you're looking for? Search the site.
Contact Syngene
Tel +44 1223 727123
Other Syngene stories
-
Dyversity aids research into human aging
The Albert Einstein College of Medicine in New York is using Dyversity, Syngene's 2D gel imaging system, to help understand the proteomic basis of human aging. -
2D gel imaging at Singapore's new Smart centre
Yngene's first Dyversity 6 2D gel imaging system has been installed at the prestigious new, Singapore MIT Alliance for Research and Technology (Smart) Centre, by distributor Insta BioAnalytik -
Save time achieving 21 CFR and GMP
Syngene has produced new software and validation information for its Dyversity, G:Box and GeneGnome range of image analysis systems -
2D gel image analyser used at Imperial College
Scientists in the Institute of Biomedical Engineering at Imperial College are using Dyversity to rapidly image 2D DIGE (differential gel electrophoresis) gels containing Cy dye labelled proteins -
Imperial is first to install Dyversity gel imager
One of the UK's premier universities, Imperial College London, has chosen Dyversity, Syngene's 2D gel imaging system, to assist in identifying the complex proteins associated with human diseases
Articles by product category
-
Analytical instruments (8,805)
- Analytical instruments (13)
- Chemical analysis equipment (631)
- Chromatographs: gas (241)
- Chromatographs: liquid, ion, gel, HPLC (427)
- Chromatography accessories (503)
- Gas analysis equipment (219)
- pH and conductivity meters (114)
- Cameras and imaging systems (820)
- Electron microscopy systems (209)
- Optical microscopes (448)
- Microscope supplies and accessories (558)
- Mass spectrometers (458)
- Spectroscopy (535)
- Other analytical instruments (592)
- Particle size analysis equipment (555)
- Microplate readers (39)
- Temperature, pressure and flow measurement (262)
- Spectrophotometers (273)
- Titration equipment (655)
- Ultrasound equipment and ultrasonic spectroscopy (118)
- Viscometers and rheometers (346)
- Oscilloscopes (83)
- Mechanical testing equipment (492)
- Test and calibration instrumentation (214)
-
Life sciences and clinical laboratory equipment (3,579)
- Life sciences and clinical laboratory equipment (3)
- Clinical chemistry analysis (898)
- Nucleic acid sequencing and synthesis (637)
- Genomics (459)
- Proteomics (450)
- Assay kits (185)
- Microbiology (383)
- Polymerase chain reaction (PCR) (240)
- Cell/tissue handling and counting (324)
-
Computer hardware (826)
- Computer hardware (7)
- Dataloggers (302)
- I/O devices (362)
- Monitors and peripherals (50)
- Process control and networking (79)
- PLCs (26)
-
Industry news (19)
- Industry news (19)
-
Laboratory equipment and wares (5,937)
- Laboratory equipment and wares (5)
- Safety and ventilation equipment (322)
- General lab equipment (831)
- Workstations (61)
- Detectors, sensors and probes (375)
- Lab automation equipment and robotics (651)
- Laboratory furniture and storage (151)
- Gas handling (125)
- Heating: ovens, furnaces, autoclaves (331)
- Fermentation and incubation equipment (73)
- Microplates/microarrays and readers (397)
- Microfluidics/Lab-on-a-chip systems (142)
- Distillation equipment (16)
- Sampling equipment (156)
- Environmental chambers (88)
- Fume cupboards ductless (79)
- Laboratory glassware (400)
- Liquid handling (415)
- Cooling: refrigeration (198)
- Washing and sterilisation equipment (207)
- Fume cupboards ducted (31)
- HTS and combinatorial chemistry equipment (57)
- Evaporation equipment (166)
- Weighing and balancing (240)
- Centrifuges (47)
- Water purification (213)
- Laboratory plasticware (160)
-
Laboratory supplies and consumables (1,271)
- Laboratory supplies and consumables (2)
- Culture media (119)
- Reagents and catalysts (169)
- Antibodies (203)
- Cleaning products (65)
- Reference standards (178)
- Chemicals and biochemicals (534)
-
Pumps, Valves, Filters (708)
- Automated Valves (66)
- Filtration equipment and supplies (176)
- Metering pumps (109)
- Other pumps (63)
- Peristaltic pumps (65)
- Vacuum pumps and compressors (144)
- Manual Valves (85)
-
Services (2,989)
- Contract research (889)
- Laboratory and scientific exhibitions and conferences (230)
- Laboratory and scientific recruitment (26)
- Laboratory and scientific consultancy services (288)
- Laboratory and scientific training and education (413)
- Laboratory building and design (50)
- Laboratory and scientific manufacturing services (136)
- Laboratory and scientific professional organisations (482)
- Laboratory and scientific books and publishing (475)
-
Computer software (2,882)
- Computer software (4)
- Chemical modelling software (181)
- Laboratory and scientific databases (327)
- Instrument control software (320)
- Laboratory information management system (LIMS) (721)
- Mathematical software (163)
- Process control and networking software (52)
- Data acquisition software (171)
- Chromatography software (284)
- Spectroscopy software (92)
- Analytical, statistical and report generating software (284)
- Data management software (283)
