Fourth new fluorescence application of Dyversity
A
Syngene product story
Edited by the Laboratorytalk editorial team
Oct 13, 2006
Dyversity can detect nanogram quantities of protein stained with Pro-Q Diamond in seconds, providing an excellent method for proteomic researchers who want to rapidly image 1D and 2D protein gels
Using a Dyversity system fitted with a Cy dye lighting module, dual wavelength transilluminator, UV and Cy3 dye emission filters, Syngene's technical experts imaged 1D acrylamide gels containing 1000-0.1ng Pro-Q Diamond stained phosphoprotein molecular weight standard, PeppermintStick (Invitrogen).
The gel images were captured using two different settings: Cy3 excitation with a Cy3 emission filter and medium-wave UV excitation with a UV emission filter.
Syngene's application specialists found both imaging conditions of Dyversity produced comparable results with identical exposure times, detecting as little as 5ng of Pro-Q Diamond stained protein in less than three seconds.
This result coupled with the fact Dyversity can also rapidly detect nanogram amounts of other fluorescent proteomics stains such as Deep Purple, Sypro Ruby and Flamingo, as well as traditional visible stains including Coomassie Blue, means the Dyversity system is an excellent, cost-effective alternative to laser scanning for imaging a variety of protein stained gels.
Laura Sullivan, Syngene's divisional manager explained: "We have designed Dyversity 6 to include a high resolution 6.3megapixel CCD camera so that unlike a laser scanner the system can simultaneously excite at the optimum fluorescent or visible light excitation peak across an entire gel, making image acquisition a task that takes seconds rather than minutes.
"Additionally, since Syngene has determined the optimal imaging conditions for detecting small quantities of protein stained with Pro-Q Diamond and a range of many other common protein dyes, Dyversity is fast becoming the perfect system for gel based proteomics research.".
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