Product category:
Nucleic acid sequencing and synthesis
News Release from: Tecan Group | Subject: Freedom Evo
Edited by the Laboratorytalk Editorial
Team on 22 November 2005
Speeding up high throughput scrapie
genotyping
Scrapie genotyping is based on a SNE assay that identifies four polymorphisms within the PRNP prion protein encoding gene affecting the susceptibility of an individual animal for the disease
The Laboratorio Central de Veterinaria in Madrid, Spain's reference laboratory for the genotyping of scrapie in sheep, is using Tecan's Freedom Evo series of robotic workstations to develop an automated, high throughput application to identify 'at risk' animals Scrapie genotyping is based on a single nucleotide extension (SNE) assay that identifies four polymorphisms within the PRNP prion protein encoding gene affecting the susceptibility of an individual animal for the disease
This article was originally published on Laboratorytalk on 7 Jul 2003 at 8.00am (UK)
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Stephan Sidler, head of the laboratory, explained: "New legislation requires all member states of the EU to establish a breeding programme to improve scrapie resistance in sheep, and genotyping is a useful way to select animals for this.
"We chose Tecan instruments because they were compatible with our 384 well format and they offered reliability, durability and flexibility to help us reach our two main goals of high throughput with reduced consumable costs.
"Primary sample distribution is done on two Freedom Evo 150s using the PosID module for sample bar code identification.
"Two Freedom Evo 200s with the Te-Mo 96 pipettor option, using both fixed steel and disposable tips, then perform the DNA extraction, PCR set-up and PCR reactions, with both PCR clean-up and enzymatic clean-up following the SNE assay done on the Te-MO 384 channel pipetting option.
"The Tecan high throughput, automated instruments mean that we have reached our goal of achieving 95% of results within two weeks.".
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